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dap and malt extract for the homebrewers

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JoYo 2020-02-15 16:44:22 -05:00
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@ -52,6 +52,7 @@ Eventually you will want to make your own cultures from your harvested mushrooms
# Sanitize Your Workspace # Sanitize Your Workspace
- [ ] empty spray bottles, chemical resistant - [ ] empty spray bottles, chemical resistant
- [ ] star san, sanitizer - [ ] star san, sanitizer
The critical time to practice good sanitation is when opening any of your containers and interacting with the spawn medium inside. The critical time to practice good sanitation is when opening any of your containers and interacting with the spawn medium inside.
@ -126,9 +127,13 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
# Culture Mediums # Culture Mediums
- [ ] food scale, grams - [ ] food scale, grams
- [ ] distilled water - [ ] distilled water
- [ ] 16 oz wide mouth mason jars with lids - [ ] 16 oz wide mouth mason jars with lids
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave) - [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
- [ ] aluminum foil - [ ] aluminum foil
imperial | metric imperial | metric
@ -138,6 +143,7 @@ imperial | metric
## Liquid Cultures ## Liquid Cultures
- [ ] light corn syrup - [ ] light corn syrup
- [ ] diammonium phosphate with urea (optional)
Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position. Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction. A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
@ -157,6 +163,8 @@ simple sugar | 15 g | 5 g
3. Add 15 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil. 3. Add 15 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour. 4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour.
5. Allow to cool and replace aluminum foil with mason jar lid. 5. Allow to cool and replace aluminum foil with mason jar lid.
@ -168,20 +176,23 @@ simple sugar | 15 g | 5 g
## Agar Slants ## Agar Slants
- [ ] malt extract agar - [ ] malt extract agar
- [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave) - [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave)
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave) - [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize. Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
This is mixed with a simple sugar like malt extract to provide mycelium calories for reproduction. When mixed with a simple sugar like malt extract this provides mycelium calories for reproduction.
Malt Extract Agar (MEA) is available premixed fairly cheap online. Malt Extract Agar (MEA) is available premixed fairly cheap online.
Mixing 2:3 grams ratio simple sugar to Agar powder is good for saving money in bulk. Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money by buying in bulk from a homebrew shop.
Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml: Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
ingredient | mason jar | ratio ingredient | mason jar | ratio
---------- | --------- | ----- ---------- | --------- | -----
water | 300 ml | 100 ml water | 300 ml | 100 ml
MEA | 15 g | 5 g agar | 9 g | 3 g
malt extract | 6 g | 2 g
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
@ -191,7 +202,7 @@ MEA | 15 g | 5 g
2. Microwave with a safe cover until boiling. 2. Microwave with a safe cover until boiling.
3. Add 15 grams of MEA to warm water and stir for 1 minute with a sanitary utensil. 3. Add 9 g of agar and 6 g of malt extract to the warm water and stir well for 1 minute with a sanitary utensil.
4. While the MEA solution is still warm, fill a sanitary syringe with the solution. 4. While the MEA solution is still warm, fill a sanitary syringe with the solution.
@ -248,6 +259,7 @@ This will allow you to walk through the methods without fear of wasting a spore
## Cloning ## Cloning
- [ ] paring knife or scalpel - [ ] paring knife or scalpel
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave) - [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
Taking mycelium samples from mushroom tissue is called cloning. Taking mycelium samples from mushroom tissue is called cloning.
@ -270,6 +282,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
## Spore Samples ## Spore Samples
- [ ] inoculation loop - [ ] inoculation loop
- [ ] flame or lighter - [ ] flame or lighter
[Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released. [Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
@ -354,11 +367,17 @@ I leave it up to you to research your mycelium species.
# Grain Spawn # Grain Spawn
- [ ] 16 oz wide mouth mason jars with lids - [ ] 16 oz wide mouth mason jars with lids
- [ ] wild bird seed with millet - [ ] wild bird seed with millet
- [ ] colander - [ ] colander
- [ ] ladle - [ ] ladle
- [ ] boiling pot - [ ] boiling pot
- [ ] aluminum foil - [ ] aluminum foil
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave) - [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
Wild bird seed is dusty and full of bacteria and molds. Wild bird seed is dusty and full of bacteria and molds.
@ -439,7 +458,9 @@ However, exposing more surface area give the mushrooms more room to sprout.
# Fruiting Chambers # Fruiting Chambers
- [ ] medium plastic tubs with lids - [ ] medium plastic tubs with lids
- [ ] coconut coir - [ ] coconut coir
- [ ] personal fan - [ ] personal fan
Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar. Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
@ -496,6 +517,8 @@ Try to maintain 90% humidity within the fruiting chamber.
- [ ] light corn syrup - [ ] light corn syrup
- [ ] diammonium phosphate with urea
- [ ] paring knife or scalpel - [ ] paring knife or scalpel
- [ ] inoculation loop - [ ] inoculation loop