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removing malt extract, it's too easy to burn.

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JoYo 2020-02-29 16:58:38 -05:00
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@ -130,23 +130,26 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
- [ ] food scale, grams
- [ ] distilled water
- [ ] 16 oz wide mouth mason jars with lids
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
- [ ] 50 to 100 ml syringes with hollow needles, [autoclavable](#autoclave)
- [ ] distilled water
- [ ] light corn syrup
- [ ] diammonium phosphate with urea (optional)
- [ ] aluminum foil
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
Imperial | Metric
-------- | ------
16 oz | 450 ml
## Liquid Cultures
- [ ] light corn syrup
- [ ] diammonium phosphate with urea (optional)
Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
@ -181,16 +184,12 @@ simple sugar | 15 g | 5 g
- [ ] agar
- [ ] light malt extract
- [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave)
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
When mixed with a simple sugar like malt extract this provides mycelium calories for reproduction.
When mixed with a simple sugar like light corn syrup this provides mycelium calories for reproduction.
Malt Extract Agar (MEA) is available premixed fairly cheap online.
Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money by buying in bulk from a homebrew shop.
Mixing 2:3 grams ratio of light corn syrup to agar powder is good for saving money by buying in bulk from a homebrew shop.
Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
@ -198,7 +197,7 @@ Ingredient | Mason Jar | Ratio
---------- | --------- | -----
water | 300 ml | 100 ml
agar | 9 g | 3 g
malt extract | 6 g | 2 g
light corn syrup | 6 g | 2 g
0. [Sanitize Your Workspace](#sanitize-your-workspace).
@ -208,7 +207,9 @@ malt extract | 6 g | 2 g
2. Microwave with a safe cover until boiling, about 1 minute.
3. Add 9 g of agar and 6 g of malt extract to the warm water and stir well for 1 minute with a sanitary utensil.
3. Add 9 g of agar and 6 g of light corn syrup to the warm water and stir well for 1 minute with a sanitary utensil.
- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
4. While the MEA solution is still warm, fill a sanitary syringe with the solution.
@ -256,8 +257,6 @@ This will allow you to walk through the methods without fear of wasting a spore
## Cloning
- [ ] paring knife or scalpel
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
Taking mycelium samples from mushroom tissue is called cloning.
@ -267,7 +266,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
- Sanitize the outside of each [culture medium](#culture-media).
1. Cut the stem off the mushroom with a sanitary knife, .
1. Cut the stem off the mushroom with a sanitary knife.
2. Pull the plunger of a sanitary syringe to halfway out.
@ -370,7 +369,7 @@ These are usually sold as
> Instant Microwavable Whole Grain Brown Rice
For your first time I recommend you try this method to learn the process.
For your first time I recommend store bought sterilized whole brown rice to learn the process without worrying about contaminated grain.
Simply cut a small hole in a corner of the sterile container and follow the instructions for [spawn inoculation](#spawn-inoculation).
Tape the small hole closed.
@ -385,8 +384,6 @@ These general instructions are the same for most [grain spawn](#grain-spawn) but
- [ ] colander
- [ ] ladle
- [ ] boiling pot
- [ ] aluminum foil
@ -414,19 +411,18 @@ Imperial | Metric
4. Bring a pot of water to a rolling boil.
5. With a ladle, carefully add one scoop at a time to the boiling water.
5. Carefully ladle scoop of the grain into the boiling water at a time until all the bird seed is added.
Let the water return to a boil for no more than 5 minutes.
6. Once all the bird seed is added let the water return to a boil.
6. Strain again with a colander for 1 hour to dry, stirring occasionally.
7. Strain again with a colander for 1 hour to dry, stirring occasionally.
7. Split evenly to each 16 oz wide mouth mason jar, about 1 cup.
8. Split evenly to each 16 oz wide mouth mason jar, about 1 cup.
8. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours.
9. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours.
9. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
10. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
11. Store in a dark cool place until inoculation.
10. Store in a dark cool place until inoculation.
# Spawn Inoculation
@ -545,25 +541,21 @@ Harvest mushrooms after their gills are exposed but before they dump their spore
- [ ] food scale, grams
- [ ] 16 oz wide mouth mason jars with lids
- [ ] distilled water
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
- [ ] 16 oz wide mouth mason jars with lids
- [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave)
- [ ] 50 to 100 ml syringes with hollow needles, [autoclavable](#autoclave)
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
- [ ] agar
- [ ] light malt extract
- [ ] light corn syrup
- [ ] diammonium phosphate with urea
- [ ] paring knife or scalpel
- [ ] agar
- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
- [ ] inoculation loop
@ -575,8 +567,6 @@ Harvest mushrooms after their gills are exposed but before they dump their spore
- [ ] colander
- [ ] ladle
- [ ] boiling pot
- [ ] aluminum foil