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JoYo 2020-08-27 17:00:59 -04:00
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@ -82,7 +82,7 @@ If you are working near your living space then a food safe sanitizer like [Star
It is very concentrate, a couple drops into a spray bottle full of water is enough.
You will know if the solution is diluted enough when it sprays easily and creates small bubbles on the sprayed surface.
It is important to note that sanitization is not sterile.
It is important to note that sanitation is not sterile.
Star San does not kill all bacteria or mold.
There is always a risk of contamination.
@ -116,7 +116,7 @@ A small culture will allow you to grow a test batch of your sample without for a
The preferred culture medium is [liquid cultures](#liquid-cultures).
[Liquid cultures](#liquid-cultures) provide a similar micro climate as agar slants with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn-and-substrate).
The disadvantage of liquid culture is they are a little difficult to identify contaminations.
The disadvantage of liquid culture is they are a little difficult to identify contamination.
You may use agar slants to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures).
This guide does not cover their use.
@ -177,13 +177,13 @@ simple sugar | 15 g | 5 g
## Autoclave
[Autoclave](https://en.wikipedia.org/wiki/Autoclave) is a generic term for pressurized steam chambers for sanitization and sterilization.
[Autoclave](https://en.wikipedia.org/wiki/Autoclave) is a generic term for pressurized steam chambers for sanitation and sterilization.
**All autoclave duration measurements use electric pressure cookers for convenience.**
If you have a stove top pressure cooker or industrial autoclave that provide a continuous 100 kPa (15 psi), you may reduce the time by 1/3 for grain spawn.
Autoclavable tools are safe to bring to 120 celsius for extended durations without damage.
Autoclavable tools are safe to bring to 120 celsius for extended duration without damage.
0. Add the steam rack to the inner pot of the electric pressure cooker.