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@ -311,19 +311,18 @@ Mycelium samples are often sold as colonized grain or wood plugs.
Usually these can be used to inoculate [grain spawn](#grain-spawn) directly. Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
Cultures are used to store these samples long-term. Cultures are used to store these samples long-term.
With sanitary tweezers place a sample in an [agar slant](#agar-slants), [liquid culture](#liquid-cultures), or grain spawn. Use sanitary tweezers to place a sample in spawn medium.
# Reading an Agar Slant # Reading an Agar Slant
[Agar slants](#agar-slants) should show growth within the first week and should be monitored for contamination weekly. [Agar slants](#agar-slants) should show growth within the first week after inoculation and should be monitored for contamination weekly.
Mycelium are snowy white with grey and blue tints as normal. Mycelium are snowy white with grey and blue tints as normal.
Any other colors and you are dealing with contamination. Any other colors and you are dealing with contamination.
Contamination is not the end of the world. Contamination is not the end of the world.
Mycelium can be isolated from a contaminated sample. If enough mycelium growth has occurred that a sample can be collected without touching the contamination then it can be transferred to fresh [agar slants](#agar-slants) and tested again.
Otherwise, a successful agar slant colony is used to create [liquid culture](#liquid-cultures).
If enough mycelium growth has occurred that a sample can be collected without touching the contamination then it can be transferred to a fresh [culture medium](#culture-mediums).
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
@ -331,15 +330,15 @@ If enough mycelium growth has occurred that a sample can be collected without to
1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool. 1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
2. Gently scrape some of the mycelium onto the loop. 2. Gently scrape some of the mycelium off of the surface of agar onto the loop.
3. Insert loop into an open [culture medium](#culture-mediums) and gently apply spores. 3. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
4. Replace the screw cap on the [culture medium](#culture-mediums) and store in ambient room light at room temperature. 4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
# Colonization # Colonization
Once the mycelium has been successfully isolated and grown in [liquid cultures](#liquid-cultures) the mycelium wants something a bit more nutritious than sugar water. Once the mycelium is successfully isolated in [liquid cultures](#liquid-cultures), the mycelium wants something a bit more nutritious than sugar water.
Most species have special requirements that encourage the growth of mushrooms. Most species have special requirements that encourage the growth of mushrooms.
Wood-loving species like oyster and shiitake thrive in a mixture of wood chips. Wood-loving species like oyster and shiitake thrive in a mixture of wood chips.
This guide does not address these special requirements. This guide does not address these special requirements.
@ -357,13 +356,18 @@ I leave it up to you to research your mycelium species.
- [ ] aluminum foil - [ ] aluminum foil
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave) - [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
Wild bird seed is rather dusty and full of bacteria and molds. Wild bird seed is dusty and full of bacteria and molds.
Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore) making it resistant to heat. Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to autoclave. Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
imperial | metric
-------- | ------
16 oz | 450 ml
1 cup | 250 ml
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
1. Fill 16 oz wide mouth mason jars with 1 cup of bird seed per final jar of grain spawn. 1. Fill 16 oz wide mouth mason jars with 1 cup of bird seed for each final jar of grain spawn.
2. Cover bird seed with tap water, lid jar, and soak for 12 to 24 hours. 2. Cover bird seed with tap water, lid jar, and soak for 12 to 24 hours.
@ -405,7 +409,7 @@ Soaking and boiling the bird seed will clean it up a bit and encourage the endos
# Reading a Spawn Jar # Reading a Spawn Jar
Similar to [Reading an Agar Slant](#reading-an-agar-slant), [grain spawn](#grain-spawn) should show growth within the first week and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks. Similar to [Reading an Agar Slant](#reading-an-agar-slant), [grain spawn](#grain-spawn) should show growth within the first week after inoculation and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks.
Mycelium are snowy white with grey and blue tints as normal. Mycelium are snowy white with grey and blue tints as normal.
@ -421,9 +425,9 @@ This is because mycelium sense that water is evaporating and wish to take advant
In our fruiting containers we want to mimic that as much as possible with a spray bottle and a light breeze. In our fruiting containers we want to mimic that as much as possible with a spray bottle and a light breeze.
This can be accomplished with an open and fully colonized [grain spawn](#grain-spawn) jar and a daily spray bottle misting. This can be accomplished by opening a fully colonized [grain spawn](#grain-spawn) jar and misting the mycelium cake with water daily.
However, if there is more surface area for the mushroom pins to sprout from then there is a higher chance of successful fruiting. However, exposing more surface area give the mushrooms more room to sprout.
# Fruiting Chamber # Fruiting Chamber
@ -433,9 +437,9 @@ However, if there is more surface area for the mushroom pins to sprout from then
Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar. Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
0. Invert an open and fully colonized [grain spawn](#grain-spawn) jar and firmly but gently tap cake out. 0. Open and invert a fully colonized [grain spawn](#grain-spawn) jar and firmly but gently tap the mycelium cake out.
- If the cake breaks up that is OK, place the pieces back together if possible. - If the cake breaks up that is OK, place the pieces back together when they are rolled in coir.
1. Soak the mycelium cake in water for 12 to 24 hours. 1. Soak the mycelium cake in water for 12 to 24 hours.
@ -443,7 +447,7 @@ Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold
3. Gently roll the mycelium cake in the hydrated coconut coir to form a light casing. 3. Gently roll the mycelium cake in the hydrated coconut coir to form a light casing.
4. Layer the bottom of the tub with an inch or more of coconut coir. 4. Layer the bottom of the tub with 5 cm or more of coconut coir.
5. Place the mycelium cake on the layer of coconut coir away from the sides. 5. Place the mycelium cake on the layer of coconut coir away from the sides.
@ -451,11 +455,11 @@ Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold
Try to maintain 90% humidity within the fruiting chamber. Try to maintain 90% humidity within the fruiting chamber.
0. With a water spray bottle gently mist the coconut coir and mycelium cake until the coir is moist but not soaked. 0. With a spray bottle of water, gently mist the coconut coir and mycelium cake until the coir is moist but not soaked.
1. Place the medium plastic tub lid upside down, slightly ajar, and rest for 1 hour. 1. Place the medium plastic tub lid upside down on the tub slightly ajar and rest for 1 hour.
2. Point a personal fan nearby to blow towards the ceiling above the tub for 1 hour. 2. Place a personal fan nearby to blow upwards towards the ceiling above the tub for 1 hour.
3. Replace the lid upside down and slightly ajar. 3. Replace the lid upside down and slightly ajar.