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					@ -1,6 +1,7 @@
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# HomeMyco
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					# HomeMyco
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A [homebrewer](https://en.wikipedia.org/wiki/Homebrewing)'s take on practical home cultivation of [mycelium](https://en.wikipedia.org/wiki/Mycelium) and their [mushrooms](https://en.wikipedia.org/wiki/Mushroom).
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					A [homebrewer](https://en.wikipedia.org/wiki/Homebrewing)'s take on practical home cultivation of [mycelium](https://en.wikipedia.org/wiki/Mycelium) and their [mushrooms](https://en.wikipedia.org/wiki/Mushroom).
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					You don't have to follow every step in this guide but you should because it's fun.
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# Always a Risk of Contamination
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					# Always a Risk of Contamination
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					@ -14,49 +15,52 @@ Contaminated spawn is easily discarded and replaced.
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As a result of the above hubris we triple our efforts.
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					As a result of the above hubris we triple our efforts.
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- When making [colonization jars](#grain-spawn) make at least two for each sample we wish to [colonize](#colonizing).
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					- Make at least two agar slants or liquid cultures for each sample we wish to test.
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- When making [agar slants](#agar-slants) or [liquid cultures](#liquid-cultures) make at least two for each sample.
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					- Make at least two colonization jars for each sample we wish to colonize.
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- Make and save at least one of the above as a control for testing your sanitation.
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					- Make one extra of the above as a control for testing your sanitation.
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It is OK.
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					It is OK.
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Seed spawn, agar, and sugar water are inexpensive.
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					Seed spawn, agar, and sugar water are inexpensive.
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					All other equipment can be reused.
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Saving a control spawn medium, both [grain spawns](#grain-spawn) and [culture mediums](#culture-mediums), will help you test for contamination introduced through your technique.
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					Saving a control spawn medium will help you test for contamination introduced through your technique.
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If all fares well you can always end up using it.
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					If all fares well you can always end up using it.
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					Spawn medium refers to any growing surface, such as grain spawn or culture mediums.
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# Finding Samples
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					# Finding Samples
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- [ ] mycelium sample
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					- [ ] mycelium sample
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At the beginning of each section is a checklist of what to buy.
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					Each section starts with a checklist of what to buy.
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At the end of the document there will be a combined checklist.
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					The end of this document has a combined checklist.
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Newbies should start with store bought mushrooms.
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					Newbies should start with grocery store mushrooms.
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This will allow you to walk through the methods without fear of wasting a sample.
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					This will allow you to walk through the methods without fear of wasting a sample.
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Getting other samples should not be difficult but it is quite frustrating to discover all if the sanitary steps were made in vain when a contaminated sample was the source.
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					Getting other samples should not be difficult but it is quite frustrating to discover all the precautions were made in vain when a contaminated sample was the source.
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Of course, there is always the risk of a contaminated store bought sample.
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					Of course there is always the risk of contaminated grocery store mushrooms.
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Online shopping can get you all sorts of sample types ranging from spawn grains and liquid culture to spores and dried mushrooms.
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					Online shopping can get you all sorts of sample types ranging from spawn grains and liquid culture to spores and dried mushrooms.
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When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate.
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					When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate.
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Eventually you will want to make your own cultures from the spores of your harvested mushrooms.
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					Eventually you will want to make your own cultures from your harvested mushrooms.
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# Sanitize Your Workspace
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					# Sanitize Your Workspace
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- [ ] empty spray bottles, chemical resistant
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					- [ ] empty spray bottles, chemical resistant
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- [ ] star san, sanitizer
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					- [ ] star san, sanitizer
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Prior to working with a mycelium sample or spawn medium you should clean your work area.
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					Clean yourself and work area before working with a mycelium sample or spawn medium.
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All surfaces and tools should be wiped clean then sprayed with a sanitizing solution at least 2 minute prior to working.
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					All surfaces and tools should be wiped clean then sprayed with a sanitizing solution to soak for at least 2 minute prior to working.
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Purchase a spray bottle [resistant to chemical reactions](https://en.wikipedia.org/wiki/Chemical_resistance) for this purpose.
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					Purchase a new spray bottle that is [resistant to chemical reactions](https://en.wikipedia.org/wiki/Chemical_resistance) for this purpose.
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These can be found at most hardware stores.
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					These can be found at most hardware stores.
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While you are at it you should buy a second spray bottle for misting water.
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					While you are at it you should buy a second spray bottle for misting water during the fruiting stage.
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If you are working near your living space a food safe sanitizer like [Star San](https://fivestarchemicals.com/star-san-sanitizer-4-oz) avoids stinking up the place or contaminating food.
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					If you are working near your living space then a food safe sanitizer like [Star San](https://fivestarchemicals.com/star-san-sanitizer-4-oz) with avoid stinking up the place or contaminating food.
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It is very concentrate so just a couple drops into a spray bottle full of water be enough.
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					It is very concentrate, a couple drops into a spray bottle full of water is enough.
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You will know if the solution is diluted enough when it sprays easily and creates small bubbles on the sprayed surface.
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					You will know if the solution is diluted enough when it sprays easily and creates small bubbles on the sprayed surface.
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It is important to note that sanitization is not sterile.
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					It is important to note that sanitization is not sterile.
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					@ -64,21 +68,22 @@ Star San does not kill all bacteria or mold.
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There is always a risk of contamination.
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					There is always a risk of contamination.
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Your face, hands, and tools are the major source of contamination.
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					Your face, hands, and tools are the major source of contamination.
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Tools can be sanitized in a pressure cooker or [autoclave](#autoclave) which is discussed later.
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					Tools can be sterilize in a pressure cooker or [autoclave](#autoclave).
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Your face, hand, and the other hand are a little more difficult to contain.
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					Your face, hand, and the other hand are a little more difficult to contain.
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While gloves and a face mask are often recommended I find they make me more clumsy to the point of negating any benefit they might provide.
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					While gloves and a face mask are often recommended, they may be more clumsy to the point of negating any benefit they might provide.
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Face masks just do not work unless they provide respiration filtering.
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					Face masks do not work unless they provide respiration filtering.
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Gloves only work if they begin sterile and remain sterile.
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					Gloves only work if they begin sterile and remain sterile.
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Scrub your hands with warm water and soap to remove anything that might fall off into the spawn medium.
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					Scrub your hands and forearms with warm water and soap to remove anything that might fall into the spawn medium.
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Avoid touching your spawn medium and the part of your tools that interact with the spawn medium.
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					Avoid touching your spawn medium directly.
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					Avoid touching the part of your tools that interact with the spawn medium.
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Wash and dry your face with warm water and soap to remove anything that might fall off into the spawn medium.
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					Wash and dry your face with warm water and soap to remove anything that might fall off into the spawn medium.
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Combing your hair and wearing a hat helps to reduce the amount of lose hair that might fall into the spawn medium.
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					Combing your hair and wearing a hat helps to reduce the amount of lose hair that might fall into the spawn medium.
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Slow or hold your breath when inoculating spawn medium to avoid shaking.
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					Slow or hold your breath when inoculating spawn medium to avoid shaking.
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Remember that mistakes happen and the look pretty cool when they do.
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					Remember that mistakes happen and they look pretty cool when they do.
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# Three Stages
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					# Three Stages
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					@ -86,7 +91,7 @@ There are three general stages to cultivating mycelium.
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1. [Culturing](#culturing), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
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					1. [Culturing](#culturing), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
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    - Using simple sugar and agar as a surface to proof sample mycelium for viability and contamination.
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					    - Using simple sugar and agar as a surface to test sample mycelium for viability and contamination.
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2. [Colonization](#colonization), [wiki](https://en.wikipedia.org/wiki/Colony_(biology)#Microbial_colonies)
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					2. [Colonization](#colonization), [wiki](https://en.wikipedia.org/wiki/Colony_(biology)#Microbial_colonies)
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# Culturing
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					# Culturing
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Any sample of mycelium is potentially contaminated.
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					Any samples of mycelium are potentially contaminated.
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Aside from using a microscope and checking the entire sample, using a small culture will allow you to grow a test patch of your sample without risking a whole jar of [grain spawn](#grain-spawn).
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					Even using a microscope and visually checking the entire sample is tedious and error prone.
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					A small culture will allow you to grow a test patch of your sample without risking a whole jar of [grain spawn](#grain-spawn).
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The two [culture mediums](#culture-mediums) preferred are [agar slants](#agar-slants) and [liquid cultures](#liquid-cultures) together.
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					The two [culture mediums](#culture-mediums) preferred are [agar slants](#agar-slants) and [liquid cultures](#liquid-cultures) together.
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While petri dishes are part of the usual trappings of cultivating the growth of microorganisms they are fairly high maintenance and better suited for a laboratory environment.
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					Agar givings mycelium and contamination a surface to grow in large enough colonies to view with the naked eye.
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Agar slants provide the same functionality without the need to seal and re-seal with parafilm.
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					A week of growth is usually long enough to produce colonies which can be identified and transferred to [liquid cultures](#liquid-cultures).
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					While petri dishes are part of the usual trappings of cultivating microorganisms, they are fairly high maintenance and better suited for a laboratory environment.
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					[Agar slants](#agar-slants) provide the same functionality without the need to seal and re-seal with parafilm.
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[Liquid cultures](#liquid-cultures) provide a similar micro climate as [agar slants](#agar-slants) with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn).
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					[Liquid cultures](#liquid-cultures) provide a similar micro climate as [agar slants](#agar-slants) with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn).
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The disadvantage of liquid culture is being unable to isolate mycelium from contaminations.
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					The disadvantage of liquid culture is they are difficult to identify contaminations.
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You should use [agar slants](#agar-slants) to isolate mycelium from contamination then sample the mycelium from the agar to liquid culture.
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					You should use [agar slants](#agar-slants) to isolate mycelium from contamination into [liquid cultures](#liquid-cultures).
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# Culture Mediums
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					# Culture Mediums
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- [ ] malt extract agar
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					- [ ] malt extract agar
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Agar agar is a plant gelatin used to provide a physical surface that mycelium can colonize.
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					Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
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This is mixed with a simple sugar like malt extract to provide mycelium calories for reproduction.
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					This is mixed with a simple sugar like malt extract to provide mycelium calories for reproduction.
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Malt Extract Agar (MEA) is available in a premix fairly cheap online.
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					Malt Extract Agar (MEA) is available premixed fairly cheap online.
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Mixing 2:3 grams ratio simple sugar to Agar powder is good for saving money in bulk.
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					Mixing 2:3 grams ratio simple sugar to Agar powder is good for saving money in bulk.
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Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
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					Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
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					|  |  |
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					-- | --
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					water | 100 ml
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					MEA | 5 g
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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					0. [Sanitize Your Workspace](#sanitize-your-workspace).
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1. Fill a 16 oz mason jar with 100 ml water on a food scale.
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					1. Fill a 16 oz mason jar with 100 ml water on a food scale.
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    - Grams is the same as milliliters of water at [STP](https://en.wikipedia.org/wiki/Standard_conditions_for_temperature_and_pressure).
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					    - Grams is the same as milliliters of water at [STP](https://en.wikipedia.org/wiki/Standard_conditions_for_temperature_and_pressure).
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2. Microwave with a safe cover for 1 minute or until 50 celsius or higher without boiling.
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					2. Microwave with a safe cover until boiling.
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3. Add 5 grams of MEA to warm water and stir for 1 minute with a sanitary utensil.
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					3. Add 5 grams of MEA to warm water and stir for 1 minute with a sanitary utensil.
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					@ -162,14 +173,17 @@ Mixing liquid culture is similar to [agar slants](#agar-slants) without needing
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A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
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					A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
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Scale the following recipe as needed to fill 2/3 of each test tube or 20 ml:
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					Scale the following recipe as needed to fill 2/3 of each test tube or 20 ml:
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					|  |  |
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					-- | --
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					water | 100 ml
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					simple sugar | 5 g
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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					0. [Sanitize Your Workspace](#sanitize-your-workspace).
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1. Fill a 16 oz mason jar with 100 ml water on a food scale.
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					1. Fill a 16 oz mason jar with 100 ml water on a food scale.
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    - Grams is the same as milliliters of water at [STP](https://en.wikipedia.org/wiki/Standard_conditions_for_temperature_and_pressure).
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					    - Grams is the same as milliliters of water at [STP](https://en.wikipedia.org/wiki/Standard_conditions_for_temperature_and_pressure).
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2. Microwave with a safe cover for 1 minute or until 50 celsius or higher without boiling.
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					2. Microwave with a safe cover until boiling.
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3. Add 5 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
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					3. Add 5 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
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					@ -197,7 +211,7 @@ Use the following durations as they make sense to sanitize and pasteurize.
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- 2 hours
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					- 2 hours
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    - Spawn grain should be autoclave for 2 hours prior to inoculation.
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					    - Spawn grain should be autoclave for 2 hours and cooled prior to inoculation.
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        Bulk grains are particularly susceptible to contamination during the commercial farming process.
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					        Bulk grains are particularly susceptible to contamination during the commercial farming process.
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- 1 hour
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					- 1 hour
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					@ -228,7 +242,7 @@ Use the following durations as they make sense to sanitize and pasteurize.
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Once you autoclave your [culture mediums](#culture-mediums) and they have cooled to room temperature it is ready for mycelium.
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					Once you autoclave your [culture mediums](#culture-mediums) and they have cooled to room temperature it is ready for mycelium.
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Depending on the source of the sample there are two ways to inoculate, [cloning](#cloning) from a mushroom or using [spore samples](spore-samples).
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					Depending on the source of the sample there are two ways to inoculate, [cloning](#cloning) from a mushroom or using [spore samples](spore-samples).
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Newbies should start with store bought mushrooms.
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					Newbies should start with grocery store mushrooms.
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This will allow you to walk through the methods without fear of wasting a spore sample.
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					This will allow you to walk through the methods without fear of wasting a spore sample.
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## Cloning
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					## Cloning
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						 | 
					@ -329,7 +343,7 @@ If enough mycelium growth has occurred that a sample can be collected without to
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# Colonization
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					# Colonization
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Once the mycelium has been successfully isolated and grown in a liquid culture the mycelium wants something a bit more nutritious than sugar water.
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					Once the mycelium has been successfully isolated and grown in [liquid cultures](#liquid-cultures) the mycelium wants something a bit more nutritious than sugar water.
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Most species have special requirements that encourage the growth of mushrooms.
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					Most species have special requirements that encourage the growth of mushrooms.
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Wood-loving species like oyster and shiitake thrive in a mixture of wood chips.
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					Wood-loving species like oyster and shiitake thrive in a mixture of wood chips.
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This guide does not address these special requirements.
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					This guide does not address these special requirements.
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						 | 
					@ -387,7 +401,7 @@ Soaking and boiling the bird seed will clean it up a bit and encourage the endos
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1. With a sanitary syringe extract 1 ml of [liquid culture](#liquid-cultures) and replace the screw top.
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					1. With a sanitary syringe extract 1 ml of [liquid culture](#liquid-cultures) and replace the screw top.
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2. Inject the liquid culture into the jar of [grain spawn](#grain-spawn) by piercing the aluminum foil straight down along the side of the jar.
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					2. Inject syringe into the jar of [grain spawn](#grain-spawn) by piercing the aluminum foil straight down along the side of the jar.
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3. Replace the mason jar lid by flipping it so the seal is facing up and lightly screwing on the rim.
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					3. Replace the mason jar lid by flipping it so the seal is facing up and lightly screwing on the rim.
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